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Changes in GnRH mRNA levels during GnRH neuronal development. Total RNA was extracted from cultures at 0, 14, and 20 div (n = 4 in all age groups). GnRH mRNA levels started to increase after 14 div, reaching the highest level at 20 div. *, P < 0.05 vs. 0 div; #, P = 0.05 vs. 14 div. GnRH mRNA levels are relative to 18s in each sample and analyzed using the ΔCT method (28).
