Table 3.

OSD genomic primers and expected PCR product sizes, designed to identify affected, carrier and normal dogs for COL9A2 and COL9A3 mutations.

#	Forward primer name	Forward primer sequence	Reverse primer name	Reverse primer sequence	Expected size in wt	Expected size in carrier	Expected size in affected
1	COL9A2_Part11F	gctgaccttgtggattttctcc	COL9A2intron1R	gtgaatgggcaccattgtct	1,445 bp	178 bp	178 bp
2	COL9A2_Part10F	catctctccctcactccctcct	COL9A2_Part10R	tcacccctctcccagtctcttag	504 bp	504 bp	No band
3	COL9A3test1F	gctgccactgggctcctttcttcg	COL9A3test1R	agcaggagcagggccagcgtg	248 bp	248 bp and 249 bp	249 bp