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. 2010 Oct 14;5(10):e13385. doi: 10.1371/journal.pone.0013385

Figure 5. Generation of inducible forebrain-specific AC8 mice on a DKO background.

Figure 5

(A) Immunohistochemistry results confirm complete absence of AC8 protein levels in DKO mice, while AC8 rescue mice have AC8 replaced within forebrain-specific regions but not in the hindbrain or thalamus. (B) Using doxycycline to manipulate AC8 expression within the forebrain, we observed AC8 expression after 2 wk off doxycycline. In contrast, expression begins to rapidly turn off within one week off doxycycline treatment and is undetectable by 2 wk. Hindbrain results reveal no AC8 expression with or without doxycycline treatment. Bip is used as a loading control. The adenylyl cyclase assays in the (C) cortex (n = 3/genotype) and (D) hippocampus (n = 3/genotype) reveal no Ca2+-stimulated activity in DKO mice or AC8 rescue mice on doxycycline, while activity is rescued to approximately 50% (C) and 30% (D) of WT levels in AC8 rescue mice off doxycycline.