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. 2010 Oct 14;5(10):e13246. doi: 10.1371/journal.pone.0013246

Figure 6. Cytogenetic Analysis.

Figure 6

Normal female, 46, XX karyotype was observed in a clonal cell line at 31.3 population doublings (A). In one flask from the same cell line, 2 out of 12 cells displayed an abnormal karyotype; a deletion on the long arm of chromosome 20 at q11.2 (B; arrowed). Telomere length analysis and real-time quantitative telomere repeat amplification procedure (RTQ-TRAP) of telomerase activity in clonal cell lines and full-depth chondrocyte populations. STELA reveals both clonal cell lines and full-depth chondrocytes undergo telomere erosion (C). It is interesting to note a subpopulation of cells in the clonal cell line show a larger distribution which is increasing with time in culture (arrowed). RTQ-TRAP analyses of telomerase activity in HL60 cell equivalents showed low population doubling (<30) clonal cells displaying a 3.1-fold greater activity than low population full depth chondrocytes (D). High population doubling clonal cells show a 10.6-fold greater activity than full depth chondrocytes at a high population doubling (>30). PD  =  population doubling.