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A, The pCIneo empty vector, T7-tagged WT and each of A- or E-Bax mutant cDNAs were transfected in Bax−/− MEF cells. After 24 h, expression levels of exogenous Bax protein were determined by Western blot using T7 antibody. B, After transfection with WT or each of Bax mutants for 48 h, cell viability was determined by analyzing annexin-V binding on FACS. Data represent the mean ± S.D. of three determinations.
