Table 1. Filter binding assays with human and B. burgdorferi Pur-α.

Protein	ssDNA 12mer	Sequence 5′ – 3′	KDs [nM]	Avg. KDs [nM]
Human Pur-α 56–287 (C272S)	hTel12	(AGG GTT)2	491, 438, 306	411±95
B. burgdorferi Pur-α 6–127	hTel12	(AGG GTT)2	435, 445, 413, 413	426±16
Human Pur-α 56–287 (C272S)	JCVupTAR	GGA GGG GGA GGC	207, 258	233±36
B. burgdorferi Pur-α 6–127	JCVupTAR	GGA GGG GGA GGC	395, 428, 521, 533	469±68
Human Pur-α 56–287 (C272S)	Control	CCT CCG CCT CCG	No binding	No binding
B. burgdorferi Pur-α 6–127	Control	CCT CCG CCT CCG	No binding	No binding

Equilibrium dissociation constants (K_D) from filter binding experiments. C272S in protein name indicates that the cysteine in amino acid position 272 was mutated to serine.