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. 2010 Oct 14;6(10):e1001159. doi: 10.1371/journal.pgen.1001159

Figure 5. Overexpression of stress-protective genes in the somatic stem cell lineages delays intestinal degeneration and limits metabolic decay.

Figure 5

A. Overexpression of Jafrac1 or Hsp68 delays age-related loss of intestinal architecture. Intestinal degeneration in aging (3, 35, and 50 days) control flies (esgGFP>+) and flies overexpressing cytoprotective genes in the ISCs (esgGFP>Jafrac1 and esgGFP>Hsp68) was scored in the posterior midgut. p- values from Pearson Xi Square test. B. Overexpression of Jafrac1 or Hsp68 under the control of esgGal4 also limits the increase in the frequency of pH3+ cells in aging intestines (Averages and SEM; Student's t-test). C. Dl expression relative to rp49 in the aging intestine measured by real-time RT-PCR (Averages and SEM; Student's t-test). D. Over-expression of Jafrac1 or Hsp68 delays age-related changes in nutrient levels. Triglycerides, free glucose and glycogens were measured in young (3–4 days old) or old (40–50 days old) flies. Concentration is shown as mg nutrient per mg fresh fly. The number at the bottom of each bar represents the number of samples. All error bars represent standard deviation, p-value from Student's t-test. E. Jafrac1 and Hsp68 expression in ISCs increases starvation tolerance in old flies. Wet starvation resistance was determined in the indicated populations of flies aged for three days (left) or for 30 days (right).