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. 2010 Oct 14;6(10):e1001148. doi: 10.1371/journal.ppat.1001148

Figure 1. Infection with different species of Leishmania inhibits AP-1 DNA binding activity.

Figure 1

(A) B10R macrophages were infected for 0.5, 1, 3 and 6 hr with L. donovani, at a ratio 20∶1 parasite/macrophage. Nuclear proteins were isolated and EMSA for AP-1 DNA binding activity was performed. A consensus DNA sequence for SP-1 binding was used as non-specific competitor (NSCO). A 100× molar excess of AP-1 probe was used as a specific competitor (SCO). (B) B10R macrophages were infected for 1 hr with L. mexicana, L. major, L donovani infantum or L. tarentolae and treated as in (A).