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. 2010 Sep 28;3:244. doi: 10.1186/1756-0500-3-244

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Copyright ©2009 Adams author et al; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Integrity and composition of the host:Brucella RNA samples pre- and post-treatment. Twenty-five (25) μg total eukaryote RNA from human (HeLa S3) and bovine (MDBK) cell lines were mixed with 2 μg of Brucella melitensis 16 M RNA (ratio 12.5:1) and treated with MICROB Enrich™(Ambion) according to the instruction manual. (A) Agarose gel electrophoresis image. Lines 1 & 2: HeLa cell line:B.melitensis 16 M RNA mix; lines 3 & 4: MDBK cell line:B.melitensis 16 M RNA mix; pre and post-treatment respectively. (B) Comparison of RNA composition from a sample of MDBK cell line:B. melitensis 16 M pre and post-treatment with MICROB Enrich™ (Ambion) and examined on an Agilent 2100 Bioanalyzer. Ribosomal RNA subunits are indicated.