Figure 4.

Fed-batch fermentation of PhTAC125 with a constant feeding of a highly-concentrated casamino acid solution (100 g l^-1). The fed-batch cultivation was performed in the Applikon fermentation system with an initial operation volume of 1.4 l and an initial stirring rate of 400 rpm at 16°C. The feeding was started after 11.02 h with a constant rate of 0.028 l h^-1. (A-B) development of growth parameters during FB-growth in the bioreactor; (A) growth curve and corresponding specific growth rate; (B) experimental data for DOT, acid and stirrer; (C-E) amino acids.