Table 1.

Quantification of allergen expression on the surface by FACS analysis

	% of expressing cells		Geometrical mean ratio
Protein	Tryptophan selection	Tryptophan and zeocin selection	Tryptophan selection	Tryptophan and zeocin selection
None	2.4	-	1.0	-
CD20	43.7 ± 1.0	74.9 ± 1.3	3.1 ± 0.2	11.5 ± 0.3
Phospholipase A1 (Ves v 1)	3.3	5.6	1.2	1.1
Hyaluronidase (Ves v 2)	5.1	5.3	1.3	1.5
Antigen 5 (Ves v 5)	32.8	73.4	2.2	9.3

The cells were stained with anti-C-myc antibody conjugated with AlexaFluor488 and analyzed by fluorescent flow cytometry. The intensity of the green fluorescent signal was measured. For calculation of percentage of expressing cells and geometrical mean ratio the labeled cells were compared with non-labeled cells. The labeling of CD-20-expressing cells was performed in triplicates and the average and standard deviation are shown.