Figure 8. IL-2 secretion by LACK-specific T cell hybridomas upon incubation with different phagocytes.

Lymphocyte-depleted cells from 4 wk-infected BALB/c mice were sorted into different cell types and the indicated numbers of cells were incubated with 10⁵ LMR7.5 T cell hybridomas with or without 1 µM of LACK_156–173. Supernatants were harvested 24 hours later and analyzed for IL-2 content by ELISA. (A) Cells were sorted into CD11c⁺ (filled circles) and CD11c⁻ (empty circles) cells. Data show the results of a typical experiment (out of 5). (B) DsRed⁺ populations: CD11c⁺ (filled circles), eosinophils (grey squares), macrophages/monocytes (empty triangles) neutrophils (filled squares). Data show results of a typical experiment (out of 3). (C) CD11c⁺ cells were sorted into DsRed⁺ cells (filled squares) and DsRed⁻ cells (filled squares). Data show results of a typical experiment (out of 4).