Table 5.
Phenotypical analysis of cells cultured in developed SFM and SCM.
| Set #1 | PBMCs (%)a | SFM (%)b | SCM (%) |
|---|---|---|---|
| CD14 | 5.21 | -c | - |
| CD19 | 20.63 | - | - |
| CD3 | 58.80 | 81.34 | 83.72 |
| CD8 | 20.42 | 30.50 | 29.86 |
| Set #2 | PBMCs (%) | SFM (%) | SCM (%) |
| CD14 | 6.66 | - | - |
| CD19 | 19.97 | - | - |
| CD3 | 62.99 | 84.32 | 88.83 |
| CD8 | 19.56 | 18.6 | 11.23 |
| Set #3 | PBMCs (%) | SFM (%) | SCM (%) |
| CD14 | 1.55 | - | - |
| CD19 | 11.53 | - | - |
| CD3 | 64.58 | 78.34 | 90.82 |
| CD8 | 19.32 | 28.58 | 6.80 |
a Freshly thawed PBMCs, before cultivation, were analyzed.
b Cell samples in the three independent cultures were taken at a maximum viable cell concentration (refer to Figure 4B).
c not detected.