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. 2007 Nov 15;40(6):835–848. doi: 10.1111/j.1365-2184.2007.00475.x

Figure 4.

Figure 4

Akt translocates to the nucleus during apoptin‐triggered cell death. (a) Localization of Akt either in the absence or in the presence of apoptin in PC‐3 cells was detected by confocal microscopy, after immunostaining with anti‐Akt antibody followed by Cy3‐conjugated secondary antibody. DAPI was used as the nuclear counterstain, and the images were overlaid (right panels). (b) Quantitative assessment of apoptin‐triggered apoptosis in NLS‐Akt expressing cells, as compared to controls. Cells were infected with adenovirus encoding NLS‐Akt, and/or were transfected to express GFP‐apoptin. Apoptotic cell death was detected by flow cytometry (Nicoletti method) 24 or 48 h after transfection. Data represent the average of three independent experiments. Significance of the data was statistically confirmed (*P < 0.02 and **P < 0.025) using Student's t‐test. Results obtained were confirmed using the MTT assay (data not shown).