Figure 4.

Distinct patterns of hindbrain foxb1.2 expression in dmbx1a and dmbx1b morphant embryos. Lateral view anterior to the left (A, C, E, G) and dorsal view anterior to the top (B, D, F, H) of 48 hpf embryos un-injected (A, B), or injected with MO1a (C, D), MO1b (E, F) or MO1a + MO1b (G, H). The mismatch control injected morpholinos resembled the un-injected controls and are not shown. (A, C, E, G) Black arrow indicates the position of the MHB, white arrowhead demarcates the position of the ventral diencephalic/hypothalamic region of the forebrain, and black arrowhead demarcate expression domain in the region of optic tectum. Black arrow in B, D, F, H demarcates the region of the rostrolateral hindbrain (where EG is located), and red arrow demarcates the expression in the medulla oblongata. MO, morpholino; MHB, midbrain-hindbrain boundary; hpf, hours post-fertilization