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. 2010 Sep 22;10:291. doi: 10.1186/1471-2148-10-291

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Copyright ©2010 Malinska et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FISH to metaphase spreads of parental diploid species (A-D) and synthetic T. miscellus lines (E-F). (A) T. pratensis 2608. (B) T. porrifolius 2607. (C) T. dubius 2613. (D) T. porrifolius 2611. (E) and (F) stand for the 111-1 and 111-4 synthetic individuals, respectively. Metaphases were hybridized with the 18S rDNA (painting 35S sites in green) and 5S rDNA (red fluorescence) probes. Note the discontinuous chromatin condensation along the loci. Regions of condensed and decondensed chromatin are interconnected with dotted lines in (A-C). Scale bar = 10 μm.