Figure 6.

FISH to metaphase spreads of synthetic T. mirus. The probe labels and scale bar are as in Figure 4. Individuals 73-14 (A, B), 70-4 (C), and 73-13 (D) had four strong plus 0-2 very weak signals (dependent on particular metaphase and sample). (B) is an expanded region of (A) showing the large locus of T. dubius origin and the minute D^po locus (arrowheads) left after the deletion of the majority of genes from the array. Lineages 98-1 (E), 134-15 (F), 116-1 (G), and 135-5 (H) showed regularly six 18S rDNA signals. The metaphase in (G) has largely decondensed A^du (arrows), partially decondensed D^po (arrowheads), and fully condensed A^po loci.