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Distribution of flotillin-1 in DRMs isolated from hippocampal neurons and SHSY5Y cells. Equal volumes of primary hippocampal lysates or SHSY5Y lysates were incubated with the indicated detergents at a final concentration of 1% (w/v) prior to fractionation by sucrose gradient centrifugation. Equal volumes of each fraction was analysed by Western blotting with an antibody to flotillin-1. Apparent molecular mass is indicated to the left.
