Table 1.
Summary of KD data for all variants of 4E-BP151–67*
 |
KD [eIF4E] (nM) |
Factor increase in KD |
| Wild type | 21 | — |
| D55E | 29 | 1.4 |
| D55S | 100 | 4.8 |
| K57E | 550 | 26 |
| K57R | 18 | 0.86 |
| K57E/E61G | 520 | 25 |
| K57E/E61K | 21 | 1.0 |
| E61K | 52 | 2.5 |
| M60E | 230 | 11 |
| M60T | 230 | 11 |
| M60A | 480 | 23 |
| R63Q | 67 | 3.2 |
| N64S | 67 | 3.2 |
| S65+P (pH 6.6) | 120 | 5.7 |
| S65+P (pH 6.9) | 160 | 7.6 |
| S65+P (pH 7.4) | 240 | 11 |
 |
KD [eIF4E (E70A)] (nM) |
Factor increase in KD |
| Wild type | 15 | — |
| S65+P (pH 7.0) | 1,600 | Not comparable to WT |
*KD values are averages calculated on the basis of data from at least two independent ITC titrations performed at pH 7.0 (or at pH specified).