Fig. 3.

Mitogenic signaling of N-oleoyl serine in osteoblasts involves Gi-protein-ERK1/2 pathway. (A) DNA synthesis in osateoblastic MC3T3 E1 cells. (B) Number of wild-type (●) and CB₂^−/− (○) newborn mouse calvarial osteoblast. (C) Inhibition of MC3T3 E1 cell DNA synthesis by pertusis toxin (PTX). (D) Stimulation of MC3T3 E1 cell Erk1/2 phosphorylation by 10⁻¹¹ M OS. (E) Inhibition of MC3T3 E1 cell number by MEK inhibitor PD98059. Data are mean ± SE obtained in three culture wells per condition. *P < 0.05 vs. Control.