Fig. 1.

AS iPSCs express markers of pluripotency and have expected karyotypes. (A) Phase-contrast (a–c) images of AS iPSCs show hESC-like morphology. Immunocytochemistry for pluripotency markers on representative iPSC lines shows expression of NANOG (d–f), SSEA4 (g–i), TRA1-60 (j–l), and TRA1-81 (m–p). (B) Karyotype analysis on G-banded chromosomes was performed by Cell Line Genetics. Representative G-banded chromosomes from two AS iPSC lines show normal chromosome counts of 46 XX (ASdel1-0) and 46 XY (ASdel2-0).