Fig. 2.

Methylation imprint at the PWS-IC is maintained during reprogramming. (A) Methylation-specific PCR analysis of genomic DNA from normal, AS, and PWS fibroblasts. Primers specific for the methylated allele amplify a band that is 174 bp, whereas primers specific for the unmethylated allele amplify a 100-bp product. Mat, maternal; Pat, paternal. (B) Methylation-specific PCR using genomic DNA from three different AS iPSC lines and two different normal iPSC lines. Neg, negative. (C) Methylation-specific PCR using genomic DNA from three different PWS iPSC lines.