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. 2010 Sep 27;107(41):17668–17673. doi: 10.1073/pnas.1004487107

Fig. 3.

Fig. 3.

Functional neurons can be generated from AS iPSCs. (A) Phase-contrast image of 10-wk-old in vitro differentiated neurons generated from AS iPSCs. (B) iPSC-derived neurons stain positively for βIII-TUBULIN (TUJ1, green). (C) S100β-positive astrocytes spontaneously arise in AS iPSC-derived neuron cultures after 6 wk of differentiation. (D) Electrophysiological recordings from a mature AS iPSC-derived neuron after 10 wk of differentiation. (Upper) Train of action potentials evoked by depolarizing current injection (50 pA). (Calibration bars: 20 mV, 0.1 s.) (Lower) Example sweeps of spontaneous EPSCs recorded from the same neuron in the absence or presence of the AMPA receptor antagonist DNQX (10 μM). (Calibration bars: 10 pA, 1 s.) (E) Electrophysiological recordings from an immature AS iPSC-derived neuron after 10 wk of differentiation. (Upper) Response to a 60-pA depolarizing current injection. (Lower) Lack of spontaneous synaptic activity. Calibration bars are the same as in D.