Fig. 4.

Translation of nonstop mRNA and NGD efficiencies of yeast Dom34 and Hbs1 mutants. The activities of Dom34 and Hbs1 mutants in nonstop mRNA translation and NGD were measured using yeast mutant strains, W303ski2Δdom34Δ or W303ski2Δhbs1Δ, harboring a plasmid expressing the indicated mutants and the p416GPDp-GFP-Rz-FLAG-HIS3 or p416GPDp-GFP-SL-FLAG-HIS3 plasmids. Detailed procedures for the activity measurements are described in Materials and Methods. Error bars, SD of three independent experiments.