Fig. 1.

The levels of Pcdhα and -γ cleavage products decrease during both neuronal development and differentiation in culture. (A and B) Western blots of endogenous Pcdhα or Pcdhγ in whole brain extracts from mice at different developmental time points. Blots were probed with polyclonal antibody against the constant region of Pcdhα (αCon) or Pcdhγ (γCon). (C and D) Western blots of endogenous Pcdhα or Pcdhγ in cell lysates from primary neurospheres cultured for 4 d in vitro and treated with the γ-secretase inhibitor DAPT (+) or vehicle control (−). (E and F) Western blots of lysates from undifferentiated (UD) and differentiated (D) CAD cells stably expressing Pcdhα4-TAP or γb7-TAP. Cells were differentiated for 0 (UD) or 72 (D) h and the blots were probed with anti-FLAG and the loading controls anti-calnexin (CNX) or anti–β-actin. *Nonspecific, cross-reacting bands; CTF1, C-terminal fragment 1; FL, full-length Pcdh. An unidentified band in E that disappears during differentiation is indicated by an arrow.