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. 2010 Sep 27;107(41):17545–17550. doi: 10.1073/pnas.1004339107

Fig. 1.

Fig. 1.

Interaction of human XPD with damaged sites in living cells. (A) Representative foci of UV damage in CHO cells detected by immunochemistry against CPDs 30 min after UV irradiation. The accumulation of XPD proteins (wild-type and K48R mutant) is visualized by measuring the fluorescence intensity in cells transfected with the respective GFP constructs. (B) Comparison between total fluorescence, reflecting the overall expression of XPD fusions, and local fluorescence intensity in UV foci (N = 30; ± SEM). See SI Text for quantification methods. (C) Dissociation of XPD-GFP proteins from UV foci determined by fluorescence recovery after photobleaching on local damage (FRAP-LD) (N = 13; ± SEM). See SI Text for a detailed description of data acquisition and analysis.