Fig. 3.
A large C-terminal deletion traps the majority of the EspP passenger domain in the periplasm. (A) Illustration of EspP Δ891–988. (B) AD202 transformed with pJH104 (Ptrc-espPΔ891–988) were treated as described in Fig. 2, and EspP-containing polypeptides were immunoprecipitated using C- and N-terminal anti-EspP antisera. The samples in B Lower were resolved on a 4–12% NuPage/MOPS gel. The percent of the passenger domain that was surface exposed or cleaved from proEspP in B is shown in C and D.