Fig. 4.

An α-helical segment traverses the EspP β barrel before the completion of passenger domain translocation. AD202 transformed with a plasmid encoding the indicated derivative of EspP*Δ1 or EspP(F963A) were pulse-labeled with [³⁵S]cysteine after the addition IPTG and subjected to a 1- or 5-min chase. Half of the cells were treated with PK, and EspP-containing polypeptides were immunoprecipitated using the C-terminal anti-EspP antisera.