Fig. 5.

Cross-linking of an early EspP secretion intermediate to BamA. AD202 were transformed with pDULEBpa and a derivative of pRI22 [P_lac-espP] harboring the F963A mutation and an amber codon at the indicated position. Cells were pulse-labeled and subjected to a 1-min chase after the addition of IPTG. Half of each sample was UV-irradiated, and equal portions were used for immunoprecipitations with the N-terminal anti-EspP antiserum or anti-BamA or anti-SurA antisera. Truncated forms of EspP that resulted from translation termination at the amber codon are denoted (*).