Table 1.
In vitro development of rhesus monkey embryo after injection of lentiviral vector
Development stage (%) |
|||||||
Group | Embryos used/monkeys* | One-cell stage | Two-cell stage | Eight-cell stage | 16-Cell stage | Morula | Blastocyst |
Four to eight cells | 12/3 | 12 | 10 (83.3) | 8 (66.7) | 6 (50.0) | 6 (50.0) | 6 (50.0) |
One or two cells | 19/3 | 19 | 17 (89.5) | 16 (84.2) | 14 (73.7) | 11 (57.9) | 10 (52.6) |
Control | 15/3 | 15 | 13 (86.7) | 10(66.7) | 10 (66.7) | 9 (60.0) | 9 (60.0) |
*The one-cell–stage embryos from three monkeys were collected and pooled. They were randomly assigned to each of three groups: one- or two-cell stage with lentiviral infection, four- to eight-cell stage with lentiviral infection, or one- or two-cell stage control group (noninjected IVF embryos). There were no significant differences in developmental rates in either experimental group or in the control group (P > 0.05).