Fig. 3.

Results for sequence analysis of 40 genomes. Four single-mutant clones (pol2-L612M, mismatch proficient) and 16 double mutants (pol2-L612M msh2Δ) were analyzed. In each case, one reference and one outgrowth genome were sequenced, representing a total of 40 genomes that are displayed side-by-side as pairs. The genome ID numbers range from 1 to 41; ID 17 is missing because it was not used for this study. (A) Plot showing the average number of reads per nucleotide (Redundancy) for each genome. The dark gray bars show redundancy for each reference genome, whereas the adjacent light gray bars show the redundancy for the paired outgrowth genome. (B) This graph depicts the number of single-base substitutions that accumulated in the genomes during the reference passage (Fig. 2, blue path), as detected by comparing the reference genome with the outgrowth genome for each clone.