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. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: Int J Cancer. 2011 Mar 15;128(6):1393–1403. doi: 10.1002/ijc.25443

Figure 3.

Figure 3

DCC and DLC1 inhibit colony forming ability, and invasion in NPC cell lines. Panel A. Colony focus assays were performed in HNE cell lines the transiently transfected with DCC and DLC1. Colonies were counted under a microscope after 2 weeks of cell incubation in the presence of G418. Panel B. Matrigel invasion was performed on equal numbers of HNE cells transfected with the DCC or DLC1 and empty vector (mock). Cells were seeded to the upper wells of Matrigel-coated chambers in serum-free medium. After incubation for 48 hours, cells in the lower chambers were stained. All experiments were done in triplicate, and values indicate means ±s.d. Statistical significance was determined using Student's two-talied t test (p values<0.05).