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. Author manuscript; available in PMC: 2011 Apr 14.
Published in final edited form as: Oncogene. 2010 Aug 2;29(41):5630–5642. doi: 10.1038/onc.2010.301

Fig. 1. Down-regulation of endogenous NPY reduces neuroblastoma growth in vitro.

Fig. 1

A. Expression of NPY was detected by real-time RT-PCR in eight tested neuroblastoma cell lines and its release confirmed by ELISA.

B. In SK-N-BE(2) cells, NPY siRNA inhibited expression of the peptide (real-time RT-PCR) and its release into the cell culture media (ELISA), which resulted in a decreased number of viable cells (MTS assay). The growth-inhibitory effect of NPY siRNA was rescued by exogenous NPY (10−7M).

(NC – negative control siRNA; NPY-1 and NPY-2 – two different siRNAs for NPY)

C. In three selected cell lines, NPY siRNA exerted growth-inhibitory effects comparable to this observed in SK-N-BE(2) cells.