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. Author manuscript; available in PMC: 2011 Apr 14.
Published in final edited form as: Oncogene. 2010 Aug 2;29(41):5630–5642. doi: 10.1038/onc.2010.301

Fig. 2. Blocking Y2Rs mimics the growth-inhibitory effect of NPY siRNA.

Fig. 2

A. Y2Rs were detected by RT-PCR and Western blot in selected neuroblastoma cell lines.

B. In SK-N-BE(2) cells, Y2R siRNA decreased Y2R mRNA (real time RT-PCR) and protein (Western blot) levels and reduced neuroblastoma cell growth (MTS assay). The growth-inhibitory effect of Y2R siRNA, was not rescued by exogenous NPY (10−7M). Co-transfection with both NPY and Y2R siRNAs augmented the effects of the single siRNAs (MTS assay).

(NT – non-transfected; NC – negative control siRNA; Y2R-1 and Y2R-2 – two different siRNAs for Y2R)

C. In all selected cell lines, 48h treatment with Y2R antagonist (BIIE0246, 10−6M) or 96h transfection with Y2R siRNA decreased the number of viable cells (MTS assay).