Table 2.

Facial morphometric analysis. P-value of significant treatment main effects (within sub-strain) for each facial measure is indicated. For the paired comparisons, (using Tukey-Kramer procedures) comparisons were made for Alc to Chow, PF to Chow, and Chow to PF groups: Bolded-typed values under columns denote that the overall treatment p-value < the corrected Bonferonni alpha = 0.0033. An asterisk (*) denotes that the Tukey-Kramer pair-wise comparison was significant (p<0.05). Gray-shaded areas are measurements with significant difference between both control groups and alcohol-exposed group.

	Measure	B6N				B6J
		Univariate				Univariate
		p value (α =0.0036)	Alc-Chow	Alc-PF	Chow-PF	p value (α =0.0036)	Alc-Chow	Alc-PF	Chow-PF
Width	minimal frontal	0.0001^*	0.001^*	1.00	0.001^*	0.30
	bitragal	0.0001^*	0.22	0.001^*	0.0001^*	0.07
	bigonial	0.0001^*	0.71	0.0001^*	0.001^*	0.92
	inner canthal	0.0001^*	0.0001^*	0.88	0.11	0.24
	outer canthal	0.0002^*	0.03^*	0.17	0.0001^*	0.09
	^{^} palpebral fissure	0.0001^*	0.0001^*	0.09	0.009^*	0.02
	whisker pad	0.0001^*	0.21	0.008^*	0.0001^*	0.0001^*	0.0001^*	0.10	0.0001^*
	columella	0.0027^*		0.004^*	0.02^*	0.75
Depth	^{^} upper face	0.0019^*	0.002^*	0.03^°	0.20	0.0001^*	0.0001^*	0.0001^*	0.80
	^{^} mid face	0.0001^*	0.0001^*	0.008^*	0.25	0.0001^*	0.001^*	0.0001^*	0.58
	^{^} lower face	0.28				0.004
Height	nasal length	0.0001^*	0.0001^*	0.0001^*	0.12	0.005^*
	nasal bridge	0.0001^*	0.0001^*	0.012^*	0.05	0.008^*
	philtrum	0.20				0.12
	lower facial height	0.80				0.002^*	0.12	0.001^*	0.35

B6N: MANOVA (n=84); Hotelling’s Trace F(30,70)=3.7, p<0.0001; Univariate adjusted alpha = 0.0033; n’s=94–113 B6J: MANOVA (n=78); Hotelling’s Trace F(30,65)=4.9, p<0.0001; Univariate adjusted alpha = 0.0033; n’s=98–118

^{^}

Measure taken on left side;

significant treatment effect, a=0.0033; *p<0.05, Tukey-Kramer;

^°

p=0.05, Tukey-Kramer.

Gray shaded area represents measurements for both Alc-PF and Alc-Chow groups that show significant differences.