Fig. 1.
Pichia pastoris shuttle vector pPpT2 (3,546 bp), P AOX1, AOX1 promoter; AOX1TT, AOX1 terminator; MCS (multiple cloning site), EcoRI, SpeI, AscI, NotI; P ILV5, promoter of the P. pastorisILV5 gene; P EM72, synthetic E. coli promoter; Zeocin Syn, synthetic Zeocin gene; AODTT, terminator of the P. pastoris AOD gene; pUC ori, origin of replication