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. 2010 Jul 28;118(1):119–127. doi: 10.1093/toxsci/kfq230

FIG. 1.

FIG. 1.

Concentration curve of APAP-induced cytotoxicity in long-term cultured PMHs. (A) Cytotoxicity of APAP in PMHs in culture for 14 days. PMHs in culture incubated for 14 days were treated with media alone or with 0, 5, 10, 20, 30, or 50mM APAP. At 20 h following APAP treatment, cells were washed to remove excess APAP and incubated with 1.5μM calcein AM (green) and 2μM ethidium homodimer (red) for 30 min. Cells were subsequently washed and visualized by fluorescence microscopy. (B) Cytotoxicity of APAP in PMHs in culture for 2, 7, or 14 days. Two-day-old PMHs were exposed to 0, 1, 5, or 10mM APAP. Higher concentrations of APAP (0, 5, 10, or 20mM) were used for 7- or 14-day-old hepatocytes. At 20 h after APAP treatment, cell viability was measured using two fluorescent dyes, calcein AM (1.5μM) and ethidium homodimer (2μM). At 30-min incubation with dyes, cells were washed in PBS and photographed by fluorescence microscopy. Original magnification is ×200.