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. 2010 Sep 1;118(1):108–118. doi: 10.1093/toxsci/kfq256

FIG. 2.

FIG. 2.

Calcium chelation suppresses GW7845-induced mitochondrial changes and apoptosis. BU-11 cells were pretreated with Vh (DMSO, 0.1%) or BAPTA (5–15μM) for 30 min and then treated with Vh (ethanol:DMSO, 50:50, 0.1%) or GW7845 (40μM). (A) Mitochondrial membrane potential was analyzed after 30 min of treatment by JC-1 staining, followed by flow cytometry. (B) Cytochrome c release was analyzed after 45 min of treatment by immunoblotting of cytoplasmic extracts from digitonin-permeablized cells. Protein expression was quantified as described in the Materials and Methods and presented as “Fold Change from Naive.” (C) Formation of cleaved active caspase-3 (17 kDa) was analyzed after 30 min of treatment by immunoblotting of cytoplasmic extracts. (D) The percentage of cells with a sub-G0/G1 DNA content was analyzed after 2 h of treatment by PI staining, followed by flow cytometry. Data are presented as means ± SE from at least three independent experiments. *Statistically different from Vh-treated (p < 0.05, ANOVA, Tukey-Kramer). **Statistically different from GW-treated alone (p < 0.05, ANOVA, Tukey-Kramer).