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. 2010 Sep 1;118(1):108–118. doi: 10.1093/toxsci/kfq256

FIG. 4.

FIG. 4.

A CaMKII inhibitor, KN93, suppresses GW7845-induced MAPK activation. BU-11 cells were pretreated with Vh (DMSO, 0.1%) or KN93 (1–5μM) for 30 min and then treated with Vh (ethanol:DMSO, 50:50, 0.1%) or GW7845 (40μM) for 30 min. Cytoplasmic extracts were prepared and analyzed for p38 MAPK activation by detection of phosphorylated p38 MAPK by immunoblotting (A), for JNK activation by in vitro kinase assay (B), and for endogenous p38 MAPK/JNK activity by detection of phosphorylated ATF-2 by immunoblotting (C). Protein expression was quantified as described in the Materials and Methods and presented as “Fold Change from Naive.” Data are presented as means ± SE from at least three independent experiments. *Statistically different from Vh-treated (p < 0.05, ANOVA, Tukey-Kramer). **Statistically different from GW-treated alone (p < 0.05, ANOVA, Tukey-Kramer).