Fig. 2.

Co-immunoprecipitation of TfGLO and TfDEF proteins. A Recombinant proteins of Trx-TfGLO and Trx-TfDEF were produced in E. coli and purified by nickel affinity chromatography. Purified proteins (0.2 μg) were separated on SDS-PAGE gel and stained with Coomassie Brilliant Blue (left; CBB). The positions of molecular mass markers are indicated on the left. The mass unit is kDa. Production of Trx fusion proteins was confirmed by immunoblot analysis using anti-Trx antibody (right; α-Trx). B TfGLO- and TfDEF-FLAG was produced by in vitro transcription/translation using rabbit reticulocyte lysate (TnT). Production of FLAG-fusion proteins was confirmed by immunoblot analysis using anti-FLAG antibody (α-FLAG). C Co-immunoprecipitation of TfGLO and TfDEF. Protein mixtures were precipitated with α-Trx, and the precipitated proteins were detected by α-FLAG