Table 2.

Differences in ferritin induction among three types of HOS cells

Cell type	Time (h)	Dose of iron (µM)
		0	75	300
HOS	6	6.69 ± 0.69c	29.74 ± 1.07a,c	34.95 ± 3.25a,c
	18	6.57 ± 0.15c	289.77 ± 50.69a,b,c	175.11 ± 34.42a,b,c
As-8w-HOS	6	2.52 ± 0.20	5.20 ± 0.67a	5.61 ± 0.58a
	18	2.18 ± 0.08	7.46 ± 0.53a	6.64 ± 0.40a
As-T-HOS	6	1.05 ± 0.53	0.87 ± 0.44	0.77 ± 0.47
	18	0.82 ± 0.43	0.92 ± 0.46	1.25 ± 0.65

Parental HOS cells, As-8w-HOS, and As-T-HOS cells were treated with different concentrations of FAC in serum-free media. Cells were lysed in RIPA buffer and ferritin was measured by ELISA. Results are presented as the means ± SE from three independent experiments and shown as ng ferritin per mg protein.

^aSignificantly different from control HOS cells (P < 0.05).

^bSignificantly different from 6-h iron treatment (P < 0.05).

^cSignificantly different from the As-8w-HOS and As-T-HOS cells under the same experimental conditions (P < 0.05).