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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Genesis. 2010 Aug;48(8):479–484. doi: 10.1002/dvg.20644

Fig. 2. A 3 kb region of the Tlx2 gene promotes endogenous gene expression within the embryos.

Fig. 2

The Tlx2-LacZ transgenic line was analyzed for expression during development and in adults. Expression of the transgene is first observed at 8.5 dpc (a) with expression restricted to the dorsal region of the closing neural tube (NT) rostrally and neural folds caudally. At 9.5 dpc (b), Tlx2-LacZ expression is seen in the condensing cranial ganglia, otic vesicle, lateral mesoderm and NT. At 10.5 dpc (c), expression begins to be detected in the DRG and SG while expression is maintained in the cranial nerves, neural tube and lateral mesoderm. In the lateral mesoderm derivatives, expression is restricted caudal to the posterior half of forelimb. At 12.5 dpc (d), expression of the transgene remains robust in cranial ganglia, DRG, SG and express can be seen in the ENS with decreased expression in the NT and lateral mesoderm. Prior to imaging, 12.5 dpc embryos were cleared in benzyl alcohol and benzyl benzoate. Expression of Tlx2-LacZ continues in adult ENS (e), adrenal gland (f), and a subset of the cells in the SG (g). ant: anterior, cn: cranial nerves, drg: dorsal root ganglia, ens; enteric nervous system, fl: forlimb, hl: hindlimb, lm: lateral mesoderm, nt: neural tube, ov: otic vesicle, post: posterior, sg: sympathetic ganglia, tg: trigeminal ganglia,