Figure 1.

Integration of sequence statistics into predictive biophysical multidomain models. The approach is implemented by first considering the three operators as DNA signals. They are used to construct a probabilistic model that provides binding scores for these and similar mutated sequences. The scores are subsequently linked parametrically to binding free energies and incorporated directly into a detailed biophysical model of transcription regulation. The link between scores and free energies is calibrated by fitting the model to a subset of experimental data. The calibrated model is then tested with different sets of data.