Table 1. Names and sequences of primers used to create TFAM mutants.
Forward and reverse primers used in construction of L58A (leucine to alanine at 58th amino acid in TFAM protein) and ΔC-25 (truncation mutant generated by the removal of 25 amino acids from C-terminal domain of full length TFAM)
| Primer name | Primer Sequence |
|---|---|
| L58A forward | 5′-CCTGTAAGTTCTTACGCTCGATTTTCTAAAGAACAACTACCC-3′ |
| L58A reverse | 5′-GGGTAGTTGTTCTTTAGAAAATCGAGCGTAAGAACTTACAGG-3′ |
| ΔC-25-forward | 5′-P-TAGAACCCAGCTTTCTTG-3′ |
| ΔC-25-reverse | 5′-P-TTGTTCTTCCCAAGACTT-3′ |