TABLE 6.
Steps of Purification and Biological Activity of F150and Acr-F4 from Weanling Rat Livera
| Material Injected | Protein (µg/rat) | DNA synthesis (cpm/mg DNA) | Specific activity (units/mg protein) | Purification-fold |
|---|---|---|---|---|
| Cytosol | 7.5 × 104 | 43,350 ± 8,820 | 0.02 | |
| OH-F | 1.0 × 104 | 66,350 ± 11,350 | 0.30 | 15 |
| 30 kDa-F | 0.27 ± 104 | 63,520 ± 13,220 | 1.05 | 52 |
| F150 | 3 | 54,380 ± 10,200 | 762 | 38,100 |
| Acr-F4 | 3 × 10−1 | 49,350 ± 7,084 | 7620 | 381,000 |
a
All data, with the exception of the ones regarding Acr-F4 (see figure 4), are from our previous publication (6). The purification scheme of HSS has been described in Table 1. The [3H]thymidine incorporation in a 40% hepatectomized rat given an injection of PBS was 16,550 ± 3000 cpm/mg DNA. The numbers are averages from no less than 20 different rats ± SD.