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. Author manuscript; available in PMC: 2010 Oct 15.
Published in final edited form as: Biochim Biophys Acta. 2007 Nov 29;1778(2):454–465. doi: 10.1016/j.bbamem.2007.11.010

Fig. 6. Mg·ATP-dependent LTC4 transport by membrane vesicles containing wild-type and mutant MRP1 proteins.

Fig. 6

Membrane vesicles prepared in Figure 5 were used to do the transport experiments. The transport experiments were performed according to the methods described in “Materials and methods”. Briefly, the assays were carried out in a 30 μl solution containing 3 μg of membrane vesicles, 10 mM MgCl2 and varying concentrations of AMP (as a control) or ATP at 37 °C for 1 min. Since the amounts of MRP1 proteins determined in Figure 5A were different, the amounts of membrane vesicles used in these experiments were adjusted to a similar amount of MRP1 by adding varying amounts of membrane vesicles prepared from parental BHK cells: 0.81 μg of wild-type MRP1 + 2.19 μg BHK; 0.82 μg of S685T + 2.18 μg BHK; 3.00 μg of S685D; 2.31 μg of S685D/D792S + 0.69 μg BHK; 1.88 μg of D792S + 1.12 μg BHK. The data are the means ± S.D. of triplicate determinations.