Table 1.
Vmax (pmol/mg/min)* | Km (μM)* | |
---|---|---|
MRP1 | 164.0 ± 7.0 | 59.0 ± 2.2 |
S685T | 330.7 ± 8.8 | 143.0 ± 8.2 |
S685D | 65.3 ± 1.2 | 249.3 ± 6.3 |
D792S | 79.3 ± 2.1 | 245.3 ± 8.2 |
S685D/D792S | 99.0 ± 2.9 | 151.3 ± 6.8 |
Km (Mg·ATP) and Vmax (LTC4) values for wild-type, S685T, S685D, D792S and S685D/D792S (n = 3) were derived from the corresponding Michaelis-Menten curves shown in Figure 6. The P value for comparison of Km (Mg·ATP) of wild-type versus S685T is 0.0001 and, therefore, the P values for other mutants must be at most 0.0001.