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. Author manuscript; available in PMC: 2011 Sep 22.
Published in final edited form as: J Am Chem Soc. 2010 Sep 22;132(37):13026–13045. doi: 10.1021/ja105485b

Figure 4. Comparison of 1H-15N correlation specta E. coli EI and EIN.

Figure 4

The 2D 15N-1H TROSY-correlation spectrum of the intact 128 kDa E. coli EI dimer (black, residues 1-573) is superimposed on the spectrum of the isolated monomeric EIN domain (red, residues 1-249) recorded at 800 MHz and 37°C. Clearly resolved cross-peaks in the EIN spectrum12 that are either not shifted or minimally shifted in intact EI are labeled, thereby permitting direct transfer of assignments of these cross-peaks from the EIN to the EI spectrum.