Figure 6.

Incorporation of PEG-PE or LPC into the liposome lipid composition inhibits TAT-induced dye leakage and lipid mixing. The liposome lipid composition was modified by replacing corresponding amounts of PC in 1), basal BMP/PC (1:1); 2), a lipid mixture with 2 mol % of PEG-PE; or 3), 20 mol % of LPC. Effects of PEG-PE and LPC incorporation on the kinetics of dye dequenching due to release of ANTS/DPX from liposomes (a), the kinetics of Rh-PE dequenching due to lipid mixing between liposomes (b), the extent of dye dequenching measured 50 min after addition of the TAT peptide (c), and the initial rate of lipid mixing (d) are shown. The concentration of TAT was 2 μM and the total lipid concentration was 25 μM. These experiments were performed in pH 5.5 buffer. Each data point in panels c and d represents the mean of three independent experiments, and error bars indicate the SD.