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Model system employed in this work involves variants of the GB domain. The sGB construct consist of the GB1 domain with a tail, L = GGSGGS, after residue 56, and a prepended sequence of Ser-GGSGGS before residue 1. The two sequence variants are sGB1 and sGB1L7I. Two types of dGB constructs were investigated: 1) dGB1L7I-(n)-GB1, which consists of sGB1L7I linked to sGB1 with an n-residue interdomain linker (n = 3, 6, 12, 18, 24); and 2) dGB3β1-(6)-GB1.
